Time-Gated Raman spectroscopy to improve chemical sensitivity for in-situ bioreactor monitoring

• Interpretation of Raman spectra is accomplished by employing chemometric models, namely partial least squares (PLS).  
• Developing and calibrating these models can be demanding, particularly in complex bioreaction systems. Calibration-free models based on linearly additive pure component spectra offer a favorable alternative, but non-linear interferences (e.g., fluorescence) reduces model performance.  
• Mitigating interference via TG-RS enables pure component model approaches. 

NIPTE and Pfizer have teamed up to demonstrate the ability of TG-RS to reduce fluorescence and improve SNR of key metabolites in bioreactions. The superior SNR will enable the development and comparison of PLS and pure component models for the quantification of these metabolites during bioreactions of industrially relevant organisms.