Blaze™ Microchip System for Real-Time Characterization of Intact Biopharmaceutical

The goal of this NIIMBL project is to align the powerful technology BlazeTM instrument system for real-time protein analysis with the most urgent needs of biopharma. This will include evaluating assay performance with Tier 1 partner samples.

Project status

100% Completed

Industry Need

The traditional two-step workflow – iCIEF followed by MS – has been cumbersome and low capacity, creating a bottleneck in protein analytics that can lead to costly development delays and bioproduction failures.

Solution

Intabio developed the Blaze Microchip iCIEF-MS System to address the critical bottleneck in protein analysis.

Outputs/Deliverables

Develop Blaze methods for six mAb drug samples. Demonstrate reproducibility and comparability with legacy partner data. Present data to partners.
Query partners on MS integration needs. Design and test additional MS adaptors.
Hold workshop with partners to assess requirements for Blaze in-line bioreactor analytics.

Impacts

Develop an in-line application for Blaze instrumentation using detection of protein structural variants by charge heterogeneity analysis with in-line mass spectrometer analysis of enable product quality assessment during bioproduction.