Focusing on IL-2 and FGF2 cytokines, Potomac Affinity Proteins teamed with the University of Maryland to scale cytokine production reliably and at a fraction of the cost. The team aimed to use Potomac Affinity Proteins’ e. Coli expression system to create a platform process for cytokine production and purification that integrates molecular machinery into traditional manufacturing technologies. The process would allow for greater scale up potential at a reduced cost, while expanding flexibility for the industry to adopt the novel purification process for other critical cytokines or proteins.
In addition to the nearly 2,000% increase over expected production, the team achieved a significant cost reduction of 90% - 99.8%, from $1k - $50k per mg to less than $100 per mg.
While initial projections were ≥50mg per run, the team was able to produce and validate 1 gram of IL-2 and FGF2 cytokines respectively from 5 Liters of cell culture.
This basic methodology can be applied across additional cytokines beyond IL-2 and FGF2, but it also can be scaled-up to manufacture significantly higher quantities, reducing cost to only dollars per mg, potentially resulting in significant manufacturing cost savings.
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Potomac Affinity Proteins
University of Maryland College Park
